Supercharged Healing: Quality and Quantity Mononuclear Cells Show Promise for Diabetic Ischemic Wounds

A new study published in International Qound Journal] (Impact Factor: 2.6 ). In this study, researchers at the Siriraj Center of Research Excellence for Systems Pharmacology (SiSP) contributed the multiplex tissue imaging analysis to study the importance of stem cell therapy, conducted by Siriraj Surgery Department.

Sheds light on a potentially powerful therapeutic approach for severe ischemic wounds in diabetic patients: the use of "Quality and Quantity" mononuclear cells (QQMNCs). Researchers investigated the efficacy of these specialized cells in promoting wound healing and preventing limb loss in a diabetic mouse model with induced ischemia.

Diabetic individuals often suffer from impaired wound healing, particularly when coupled with poor blood flow (ischemia). This can lead to chronic, non-healing ulcers and, in severe cases, the need for limb amputation. The quest for effective treatments to accelerate healing and salvage limbs is therefore critical.

This study employed female BALB/c nude mice made diabetic to mimic this challenging clinical scenario. The researchers created ischemic wounds and then compared the effects of intramuscular injections of human QQMNCs against two control groups: one receiving standard phosphate-buffered saline (PBS) and another receiving peripheral blood mononuclear cells (PBMNCs), a more conventional cell therapy approach.

In conclusion, this preclinical study provides compelling evidence for the effectiveness of QQMNCs in promoting wound healing and preventing severe limb loss in a diabetic ischemic animal model. The enhanced regenerative and immunomodulatory properties of QQMNCs, as evidenced by their cellular composition and the positive outcomes in the mice, suggest a promising avenue for treating this challenging clinical problem.

Published in international wound journal (Q1 Impact Factor: 2.6 )
Article DOI : 10.1111/iwj.70106

Citation: Sriphan P, Chruewkamlow N, Sathan-ard C, Phutthakunphithak P, Sampattavanich S, Anekpuritanang T, et al. Effectiveness of quality and quantity mononuclear cells for enhancing wound healing in diabetic ischemic limb animal model. Int Wound J [Internet]. 2025 Apr 7 [cited 2025 Apr 10]; Available from: https://doi.org/10.1111/iwj.70106